Fluid resuscitation of acute hemorrhage in uninephrectomized rabbits: effects on the early function of the remnant kidney

The aim of this study was to determine the effects of fluid resuscitation of acute hemorrhage on the early function and histopathology of the remnant kidney in uninephrectomized rabbits. Thirty-nine adult rabbits were studied in four groups. Group I [n = 8] included healthy controls; Group 2 [n = 10] healthy, bled animals; Group 3 [n = 10] uninephrectomized, non-bled animals; and Group 4 [n = 11] uninephrectomized, bled animals. In the hemorrhage groups, 8 mL kg[-1] of blood was drawn, and replaced with lactated Ringer's solution three times the volume of shed blood. Urine and blood samples were collected after 120-minutes of observation. None of the animals experienced hypotension during the study period. Serum and urinary electrolytes were similar between the Groups [p > 0.05]. Urine output was lower in Groups 3 and 4 than in Group 1 [p=0.001, both]. Urinary microalbumin, NAG, fractional sodium excretion and creatinine clearance were similar in all four Groups. Light microscopic evaluation revealed only slight enlargement of the proximal tubule lumen in the renal medulla of the rabbits that were both uninephrectomized and bled. We observed no deleterious effects of well resuscitated hemorrhage on early function and histopathology of the remnant kidney in uninephrectomized rabbits

Structural and immunophenotypic characterization of high endothelial venules in rat and human tissue

To present additional data on high endothelial venule [HEV] structure and immunophenotype. We used the zinc iodide-osmium tetroxide technique [ZIO], which is a metallophilic fixation and staining technique to examine HEVs at light and electron microscopic levels as this technique was previously reported to be reactive with cells in HEVs. Tonsils and lymph nodes were obtained from the Surgery and Otorhinolaryngology Departments, Hacettepe University Hospital, Ankara, Turkey during 2002 and 2003. An indirect immunohistochemical technique was used to examine frozen human tissue samples. Organelle rich high endothelial cells, sheet-like processes of pericytes surrounding HEVs, structural relation of pericyte processes with fibroblastic reticular cells, an unusual multivesicular body-like organelle within high endothelial cells were presented. Expression of a large panel of defined and yet non-defined antigens on HEVs are also presented using an indirect immunoperoxidase technique. Presence of some of these antigens on HEVs was previously reported while no previous report is available for others. Significance of the expression of these antigens in HEVs, structural hints for trans endothelial migration of lymphocytes and their travel along the reticular cell meshwork is briefly discussed

Fibroblastic reticular cells and fibroblast-like cells determined by monoclonal antibodies B-F45 and B-D46 in humans

Identification of stromal microenvironmental components of lymphoid organs is relatively harder at light microscopic level as few markers, which are mostly not very specific, are available to be used for such a purpose. We screened a large panel to determine monoclonal antibodies [mAbs] those reactive with fibroblasts/fibroblast-like cells aiming to obtain further evidence for the organization and function of this cell group. Tissue samples of forty patients undergoing surgery in Otorhinolaryngology, Obstetrics and Gynecology, Orthopedics and Traumatology, Cardiovascular Surgery and General Surgery Departments, Hacettepe University Medical Faculty Hospital, Ankara, Turkey, due to different pathologies obtained as partial specimens of surgery which were apart from pathological examination were immunostained by indirect immunoperoxidase method in Histology and Embryology department in 2003. Among the screened monoclonal antibodies, monoclonal antibodies B-F45 and B-D46 reacted with the members of the family, therefore examined in detail in available human organs. Among the unique staining patterns of these mAbs, reactivity on fibroblastic reticular cells, perineural sheet cells pericryptal/perivillous fibroblasts were striking. Both mAbs will provide useful tools for further studies on stromal network of peripheral lymphoid organs and peripheral nerves